[1]谢秀利,林惠彬,齐肖,等.DEP对凡纳滨对虾NAGase活力及mRNA表达量的影响[J].泉州师范学院学报,2018,(02):1-5,21.
 XIE Xiuli,LIN Huibin,QI Xiao,et al.Effects of Diethyl Phthalate Exposure on the Activity and mRNA Expression of N-Acetylglucosaminidase from L.vannamei[J].,2018,(02):1-5,21.
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DEP对凡纳滨对虾NAGase活力及mRNA表达量的影响()
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《泉州师范学院学报》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
1-5,21
栏目:
生命科学
出版日期:
2018-04-15

文章信息/Info

Title:
Effects of Diethyl Phthalate Exposure on the Activity and mRNA Expression of N-Acetylglucosaminidase from L.vannamei
文章编号:
1009-8224(2018)02-0001-05
作者:
谢秀利1 林惠彬2 齐肖1 谢晓兰1*
1.泉州师范学院 化工与材料学院,福建 泉州 362000; 2.惠安县净峰镇政府,福建 惠安 362100
Author(s):
XIE Xiuli1LIN Huibin2QI Xiao1XIE Xiaolan1*
1.College of Chemical Engineering and Materials,Quanzhou Normal University,Fujian 362000,China; 2.Jingfeng Town Government of Hui'an County, Fujian 362100,China
关键词:
邻苯二甲酸二乙酯 凡纳滨对虾 N-乙酰氨基葡萄糖苷酶 酶比活力 酶mRNA表达量
Keywords:
diethyl phthalate L.vannamei N-acetylglucosaminidase enzyme activity enzymemRNA expression
分类号:
S949
文献标志码:
A
摘要:
采用酶促反应动力学法及实时荧光定量PCR检测分析养殖水体邻苯二甲酸二乙酯(DEP)暴露对凡纳滨对虾NAGase的比活力和mRNA表达量的影响.结果显示,10 μg/L DEP暴露120 h后,壳膜NAGase比活力出现显著性下降,但暴露至240 h后,壳膜NAGase比活力又回升至对照组水平; DEP暴露让内脏NAGase的比活力出现先升后降趋势,但趋势不显著.DEP暴露120h后,壳膜NAGase的mRNA表达量随DEP浓度增大而增大,20 μg/L DEP使mRNA表达量显著性升高; 暴露延长至240 h后,壳膜NAGase的mRNA表达量又被回调.10 μg/L DEP暴露也会激活内脏NAGase的mRNA表达,且激活程度随着暴露时间的延长而提高、激活趋势由不显著转化为显著.
Abstract:
Diethyl phthalate(DEP)is a common organic pollutants in water and has endocrinedisruptors and mutagenic effect.In this paper,the effects of DEP exposure on the activityand mRNA expression of N-acetylgulcosaminidase(NAGase)from L.vannamei were investigated byusing enzymatic reaction dynamic method and the real-time fluorescent quantitative PCR.Theresults showed that the activity of epidermal NAGase was significantly declined after prawns(L.vannamei)were exposed to the aquatic water containing 10 μg/L DEP for 120 h,but thatwas recovered to the normal levels after being exposed for 240 h.DEP exposures firstly madevisceral NAGase activity increase, and then descend.However,the trend of change of thevisceral NAGase activity was not significant.Accompanied by the increase of DEPconcentrations,the mRNA expressions of epidermal NAGase also increased while the prawnswere exposed to DEP for 120 h.The 20 μg/L DEP made the mRNA expression of epidermal NAGase enhanced significantly.The 10 μg/L DEP also made the mRNA expression of visceral NAGase activated significantly after the prawns were exposed to DEP for 240 h.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-10-31
通信作者:谢晓兰(1974-),女,福建惠安人,教授,博士,从事生物化学及生态毒理学研究,E-mail:xxl_qztc@163.com.
基金项目:国家自然科学青年基金项目(31302190); 福建省自然科学基金项目(2012J01047); 泉州市优秀人才培养专项(16D04)
更新日期/Last Update: 2018-04-15