[1]董乐,李慧珍,鲍吉雅泰,等.球形棕囊藻对营养素的响应及其多糖抗氧化活性[J].泉州师范学院学报,2018,(02):27-31,66.
 DONG Le,LI Huizhen,BAOJI Yatai,et al.Response of Phaeocystis Globosa Scherffel to Nutrient Concentrations and Antioxidant Activity of Polysaccharides from P.Globosa[J].,2018,(02):27-31,66.
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球形棕囊藻对营养素的响应及其多糖抗氧化活性()
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《泉州师范学院学报》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
27-31,66
栏目:
生命科学
出版日期:
2018-04-15

文章信息/Info

Title:
Response of Phaeocystis Globosa Scherffel to Nutrient Concentrations and Antioxidant Activity of Polysaccharides from P.Globosa
文章编号:
1009-8224(2018)02-0027-05
作者:
董乐123李慧珍123鲍吉雅泰123戴聪杰123*李元跃4黄卫红123
1.泉州师范学院 海洋与食品学院,福建 泉州 362000; 2.泉州师范学院 福建省海洋藻类活性物质制备与功能开发重点实验室,福建 泉州 362000; 3.泉州师范学院 近海资源生物技术福建省高校重点实验室,福建 泉州 362000; 4.集美大学 福建省海洋渔业资源与生态环境重点实验室,福建 厦门 361000
Author(s):
DONG Le123LI Huizhen123BAOJI Yatai123DAI Congjie123*LI Yuanyue4HUANG Weihong123
1.College of Ocean and Food Science,Quanzhou Normal University,Fujian 362000,China; 2.Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae,Fujian 362000,China; 3.Key Laboratory of Inshore Resources and Biotechnology,Fujian Province University,Fujian 362000,China; 4.Key Laboratory of Marine Fisheries Resources and Environment of Fujian Province,Fujian 361000,China
关键词:
球形棕囊藻 分离 纯化 营养素 多糖 抗氧化
Keywords:
Phaeocystis Globosa scherffel isolation purification nutrient polysaccharide antioxidant activity
分类号:
X522
文献标志码:
A
摘要:
探讨在温度、光照一定时,无机氮、无机磷和维生素对球形棕囊藻生长的影响,为揭示球形棕囊藻赤潮形成机理提供实验数据.评价球形棕囊藻多糖抗氧化活性,以探究其在食品、医药行业中的应用价值.采用平板法分离纯化并培养球形棕囊藻,利用分光光度计和称重法对经不同浓度无机氮、无机磷和维生素等营养素处理的球形棕囊藻的生物指标进行测定,同时以球形棕囊藻多糖对超氧阴离子自由基(O2-·)和羟基自由基(·OH)清除能力为指标,评价其抗氧化活性.球形棕囊藻生长的最适NaNO3和NaH2PO4浓度分别为0.112 5和0.007 5 mg/L,最适维生素B1、维生素B12和生物素浓度分别为15,7.5和75 mg/mL; 球形棕囊藻多糖对O2-·、·OH具有一定的的清除能力,对O2-·和·OH的半清除浓度(IC50)值分别为6.621 0和3.790 0 mg/mL,不如维生素C的清除效果.无机氮、无机磷和维生素对球形棕囊藻生长有显著的影响,球形棕囊藻多糖具有一定的抗氧化活性.
Abstract:
The present study explored the effects of inorganic nitrogen,inorganic phosphorus and vitamin concentrations on growth dynamic characteristics of Phaeocystis globosa Scherffel under a constant temperature and light-dark cycles in order to provide basis for understanding the mechanisms behind occurrence of red tide, evaluated the antioxidant activity of polysaccharides from P.Globosa for the prediction of the application value of P.Globosa in the food and drug industry.Through the spread plate method and streak plate method,P.Globosa was separated.Growth dynamic characteristics of P.Globosa, as a function of inorganic nitrogen, inorganic phosphorus and vitamin concentrations,were examined in a 0.2 L bioreactor with batch cultures, through spectrophotometer and weighing method.The antioxidant activities of the polysaccharides from P.Globosa were evaluated by using superoxide anion free radical(O2-·)and hydroxyl free radical(·OH)-scavenging assays.The concentrations of inorganic nitrogen and inorganic phosphorus for optimal growth of P.Globosa were 0.112 5 g/L and 0.007 5 g/L respectively,the concentrations of vitamin B1, vitamin B12 and biotin were 15 mg/mL,7.5 mg/mL and 75 mg/mL.The polysaccharides from P.Globosa had O2-· and ·OH radicals scavenging ability, the half-scavenging concentration IC50 values of the polysaccharides from P.Globosa against O2-· and ·OH radicals were 6.621 0 mg/mL and 3.790 0 mg/mL respectively,and lower than that of vitamin C.Inorganic nitrogen, inorganic phosphorus and vitamin have a significant effect on growth dynamic characteristics of P.Globosa.The polysaccharides from P.Globosa have the antioxidant activity.

参考文献/References:

[1] ROUSSEAU V,JACOBSEN A,VERITY P,et a1.The life cycle of phaeocystis:state of knowledge and presumptive role in ecology[J].Biogeochemistry,2007,83:29-47.
[2] NEJSTGAARD J C,KAM W T,STEINKE M,et a1.Zooplankton grazing on phaeocystis:a quantitative review and future challenges[J].Biogeochemistry,2007,83:147-172.
[3] WANG X,WANG Y,SMITH W O.The role of nitrogen on the growth and colony development of Phaocystis globosa(Prymnesiophyceae)[J].European Journal of Phycology,2011,46:305-314.
[4] 沈萍萍,王艳,齐雨藻,等.球形棕囊藻的生长特性及生活史研究[J].水生生物学报,2000,24(6):635-643.
[5] 尹伊伟,王朝晖,江天久.海洋赤潮毒素对鱼类的毒害[J].海洋环境科学,2000,19(2):62-65.
[6] VELDHUIS M J W,COLIJN F,ADMIRAAL W.Phosphate utilization in phaeocystis pouchetii(haptophyceae)[J].Marine Ecology,1991,12(1):53-62.
[7] SANJEEWA K K A,LEE J S,KIM W S,et a1.The potential of brown-algae polysaccharides for the development of anticancer agents:an update on anticancer effects reported for fucoidan and laminaran[J].Carbohydrate Polymers,2017,177:451-459.
[8] XU S Y,HUANG X,CHEONG K L.Recent advances in marine algae polysaccharides: isolation,structure,and activities[J].Marine Drugs,2017,15(12):388-403.
[9] 卫生部政策法规司.中华人民共和国食品安全国家标准汇编:食品中水分的测定GB 5009.3-2016[S].北京:中国标准出版社,2016.
[10] 郝继伟.超声法提取蒙山松菇多糖的工艺研究[J].食品工业科技,2011,32(1):213-218.
[11] 杨卫,王承明.超声水提花生粕多糖工艺的研究[J].中国油脂,2010,35(2):60-63.
[12] 陈红,王大为,李侠,等.不同方法提取大豆多糖的工艺优化研究[J].食品科学,2010,3(4):6-10.
[13] DUBOIS M,GIUES K A,HAMILTON J K,et a1.Colorimetfic mehod for detenninmion of sugars and related substances[J].Analytical Chemistry,1956,28:350-356.
[14] 韩少华,朱靖溶,王妍妍.邻苯三酚自氧化法测定抗氧亿活性的方法研究[J].中国酿造,2009(6):155-157.
[15] 齐亚娥,吴冬青,安红钢,等.玫瑰花蕾黄酮类化合物提取及清除羟自由基能力[J].食品科技,2007,32(4):82-85.

备注/Memo

备注/Memo:
收稿日期:2017-11-03
通信作者:戴聪杰(1966-),男,福建南安人,教授,博士,主要从事海洋生物学及天然产物研究,E-mail:congjiedai501@163.com.
基金项目:福建省教育厅科技计划项目(JK2013039); 福建省引导性科技项目(2014N0017,2015N0014); 泉州市“海纳百川”高端人才聚集计划海洋人才引进资助项目(2013); 福建省海洋渔业资源与生态环境重点实验室开放基金(fmfre2014008); 国家级大学生创新创业训练计划项目(201510399022)
更新日期/Last Update: 2018-04-15