[1]吕凤娇,赖燕霞.载黄芩苷聚乙烯吡咯烷酮纳米胶束的制备[J].泉州师范学院学报,2018,(06):1-6.
 L Fengjiao,LAI Yanxia.Preparation Process of Polyvinylpyrrolidone Nano-micelle Containing Baicalin[J].,2018,(06):1-6.
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载黄芩苷聚乙烯吡咯烷酮纳米胶束的制备()
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《泉州师范学院学报》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年06期
页码:
1-6
栏目:
化学?生命科学
出版日期:
2018-12-15

文章信息/Info

Title:
Preparation Process of Polyvinylpyrrolidone Nano-micelle Containing Baicalin
文章编号:
1009-8224(2018)06-0001-06
作者:
吕凤娇赖燕霞
泉州师范学院 化工与材料学院,福建 泉州 362000
Author(s):
LÜ FengjiaoLAI Yanxia
School of Chemical Engineering and Materials Science,Quanzhou Normal University,Fujian 362000,China
关键词:
纳米胶束 黄芩苷 聚乙烯吡咯烷酮
Keywords:
nano-micelle polyvinylpyrrolidone baicalin
分类号:
R944
文献标志码:
A
摘要:
用薄膜分散法制备载黄芩苷的大豆卵磷脂-十二烷基硫酸钠-聚乙烯吡咯烷酮K30三元混合物纳米胶束,研究其制备工艺.利用超滤离心法分离游离黄芩苷,在紫外分光光度下测定胶束中黄芩苷的含量并计算其包封率.通过单因素实验考察黄芩苷与大豆卵磷脂比例、聚乙烯吡咯烷酮K30投量、十二烷基硫酸钠投量、黄芩苷投量以及水浴温度等因素对黄芩苷纳米胶束包封率的影响,筛选出影响显著的因素.采用L9(34)正交实验设计进一步确定最优处方条件:黄芩苷与大豆卵磷脂比例为1:40,十二烷基硫酸钠投量为0.20 g,聚乙烯吡咯烷酮投量为0.20 g,黄芩苷投量为3.0 mg.该条件下,黄芩苷纳米胶束的包封率可达(92.62±1.77)%.激光粒度分析仪结果显示,黄芩苷纳米胶束平均粒径为(188.1±3.37)nm,PDI为0.163,粒径分布较为均匀.透射电镜结果显示,黄芩苷纳米胶束的形态多呈圆形或类圆形,大小与激光粒度分析所得粒径范围基本吻合.
Abstract:
Preparation of baicalin nano-micelle was studied,soybean lecithin-sodium dodecyl sulfate-polyvinylpyrrolidone K30 ternary mixtures were prepared by film dispersion method,separating the free drugs by ultracentrifugation and determing the encapsulation rate by UV spectrophotometry.Based on the results from single factor tests,orthogonal experimental design method was used to investigate the factors influence on envelopment rate of liposome. In this experiment, the optimum conditions for the preparation of nano-micelles were as follows:the ratio of baicalin and soybean lecithin 1:40, sodium dodecyl sulfate dosage 0.20 g,polyvinyl pyrrolidone dosage 0.20 g,baicalin dosage of 3.0 mg.The mean diameter was(188.1±3.37)nm,PDI 0.163,and encapsulation rate(92.62±1.77)%.The method could be used to prepare baicalin nano-micelle of soybean lecithin-sodium dodecyl sulfate-polyvinylpyrrolidone K30.

参考文献/References:

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相似文献/References:

[1]高平章,曾炜,黄金水,等.pH对黄芩苷与牛血清白蛋白相互作用的影响[J].泉州师范学院学报,2013,(06):9.
 GAO Ping-zhang,ZENG Wei,HUANG Jin-shui,et al.Effects of pH on the Interaction of Baicalin with Bovine Serum Albumin[J].,2013,(06):9.

备注/Memo

备注/Memo:
收稿日期:2018-08-10 作者简介:吕凤娇(1982-),女,福建泉州人,副教授,硕士,从事新型药物材料与剂型研究. 基金项目:泉州市科技项目(2018Z021); 大学生创新训练项目(201710399043)
更新日期/Last Update: 2018-12-15