[1]颜静宛,林智敏,苏军,等.一个水稻Bowman-Birk家族基因功能的研究[J].泉州师范学院学报,2018,(06):20-24.
 YAN Jingwan,LIN Zhimin,SU Jun,et al.A Preliminary Study on the Functions of a Rice Bowman-Birk Family Gene[J].,2018,(06):20-24.
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一个水稻Bowman-Birk家族基因功能的研究()
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《泉州师范学院学报》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年06期
页码:
20-24
栏目:
化学?生命科学
出版日期:
2018-12-15

文章信息/Info

Title:
A Preliminary Study on the Functions of a Rice Bowman-Birk Family Gene
文章编号:
1009-8224(2018)06-0020-05
作者:
颜静宛林智敏苏军宋亚娜胡太蛟王锋*
福建省农业科学院生物技术研究所 农业遗传重点实验室,福建 福州 350003
Author(s):
YAN JingwanLIN ZhiminSU JunSONG YanaHU TaijiaoWANG Feng*
Key Laboratory of Agricultural Genetics,Institute of Biotechnology of Fujian Academy of Agricultural Sciences,Fujian 350003,China
关键词:
水稻 Bowman-Birk 基因克隆 功能分析
Keywords:
Oryza sativa Bowman-Birk gene clone functional analysis
分类号:
Q789
文献标志码:
A
摘要:
采用荧光定量分析和过量表达的方法研究水稻Bowman-Birk家族基因BBTI-13基因的功能.结果显示,BBTI-13在野生型日本晴水稻中具有组织特异性,在胚和花药中表达最低; BBTI-13基因的烟草亚细胞表达定位表明其为组成型表达; 过表达株系的田间表型分析发现,过表达转基因植株与野生型相比株型矮化,粒型稍变大.并通过对水稻株形矮化相关基因的表达进一步分析表明,BBTI-13基因的过量表达会引起水稻相关矮化基因的上调和下调反馈,其中Ghd2、DTH7、Ghd7、THIS1和Ghd8表现上调,OsBZR1、OsSIN表现下调.
Abstract:
The function of BBTI-13 gene which belonging to Bowman-Birk family in rice was studied by fluorescence quantitative analysis and gene overexpression. The results showed that BBTI-13 was tissue-specific expression in the wild-type rice,with the lowest expression in embryos and anthers.The subcell localization of BBTI-13 in tobacco indicated that BBTI-13 was constitutively expressed.The phenotypic analysis of over-expressed lines in field showed that the overexpressed transgenic plants were shorter than the wild-type rice,and the grain size was slightly bigger. Further analysis showed that overexpression of BBTI-13 gene could cause up-regulation and down-regulation feedback for rice dwarf-related genes,for example,Ghd2, DTH7, Ghd7, THIS1 and Ghd8 were up-regulated,while OsBZR1 and OsSIN were down-regulated.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-07-26 通信作者:王锋(1963-),男,湖北桃江人,研究员,博士,从事植物基因工程研究,E-mail:wf@fjage.org. 基金项目:福建省属公益类科研院所基本科研专项(2015R1019-14); 福建省自然科学基金(2016J01131)
更新日期/Last Update: 2018-12-15