[1]王冰梅,钟峰波,付旺,等.WNT3、FZD4、TCF4、DKK1基因和CTNNB1蛋白在人舌早期发育中的表达[J].泉州师范学院学报,2020,(02):24-30.
 WANG Bingmei,ZHONG Fengbo,FU Wang,et al.An Expression Patterns of WNT3、FZD4、TCF4、DKK1 and CTNNB1 during Human Tongue Development[J].,2020,(02):24-30.
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WNT3、FZD4、TCF4、DKK1基因和CTNNB1蛋白在人舌早期发育中的表达()
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《泉州师范学院学报》[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年02期
页码:
24-30
栏目:
化学·生命科学
出版日期:
2020-04-15

文章信息/Info

Title:
An Expression Patterns of WNT3、FZD4、TCF4、DKK1 and CTNNB1 during Human Tongue Development
文章编号:
1009-8224(2020)02-0024-07
作者:
王冰梅12钟峰波12付旺12张超12曾金镇12郝梦瑶12
1.福建省发育与神经生物学重点实验室,福建 福州 350117; 2.福建师范大学 生命科学学院,福建 福州 350117
Author(s):
WANG Bingmei12ZHONG Fengbo12FU Wang12ZHANG Chao12ZENG Jinzhen12HAO Mengyao12
1.Fujian Key Laboratory of Developmental and Neuro Biology; Fuzhou Fujian 350117,China; 2.College of Life Sciences,Fujian Normal University,Fuzhou Fujian 350117,China
关键词:
Wnt/β-catenin信号通路 人舌 发育 表达模式
Keywords:
Wnt/β-catenin signaling human tongue development expression pattern
分类号:
Q257
文献标志码:
A
摘要:
Wnt/β-catenin信号通路在小鼠舌发育的过程中起着重要的作用,但是在人舌早期发育过程中的作用研究未见报道.该研究以人舌胚为材料,通过RNA原位杂交技术和Real-time PCR技术检测Wnt/β-catenin信号通路的关键因子(配体WNT3,受体FZD4,转录因子TCF4,抑制因子DKK1)在人舌胚早期发育过程中的表达模式.结果显示,WNT3、FZD4、TCF4和DKK1基因均在9 周和12 周人舌胚的上皮和舌肌中表达,而在舌间充质未见表达.利用免疫荧光技术验证结果显示,Wnt/β-catenin信号通路的关键转导因子(CTNNB1蛋白)在人舌胚的上皮表达.研究表明,Wnt/β-catenin信号通路的关键因子WNT3、FZD4、TCF4、DKK1和关键转导因子CTNNB1通过在舌上皮表达参与调控人舌胚发育过程.
Abstract:
Wnt/β-catenin signaling components play an important role in the development of mouse tongue, but little is known about the situation of these molecules in human embryonic tongue. In this study, expression patterns of Wnt/β-catenin signaling pathway critical factors(WNT ligand WNT3,receptor FZD4, transducer TCF4 and antagonist DKK1)were detected in early development human tongue by in situ hybridization. The results showed that WNT3, FZD4,TCF4 and DKK1 were examined in the epitheliums and muscles of tongue,in which WNT3 and DKK1 showed slightly stronger localization.And then,CTNNB1 protein was also detected in the epitheliums of the tongue by immunofluorescence. The results revealed the status of active WNT3,FZD4,TCF4,DKK1 and CTNNB1 signaling in the developing human tongue.

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备注/Memo

备注/Memo:
收稿日期:2020-02-01
作者简介:王冰梅(1982-),女,福建省南安人,副教授,博士,从事干细胞与器官发育生物学研究.
基金项目:福建省自然科学基金项目(2016J01144); 福建省教育厅科技项目(JAT160103); 福建师范大学“溪源江学者”项目(FZSKG2018017)
更新日期/Last Update: 2020-04-15